I am doing a lab on the spectroscopic analysis of antioxidant activity in herbal extracts. The lab has the initial concentrations of the extracts, and in the lab different L are added to the radical DPPH (kept constant) and absorbance values are then tested using a spectrophotometer. The question I have is that when I am plotting the data, I need the concentration per cuvette ( g/mL) for the herbal extracts. But I don’t understand how the concentrations will vary if different quantities ( L) were tested. Even though the lab says to convert molarity of stock solution to g/ml, and that each of the concentrations should correspond with the extracts. Does the concentration vary because of the different quantities of the stock solutions placed? And how would I solve for the concentrations then?
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